Research Title: Novel Antibiotics and Plant Extract combination against Infection with Escherichia coli and Staphylococcus aureus

Author: Rebwar M. Hama Salih, Beriwan Abdulaqadir Ali and Karzan Abdulmuhsin Mohammad

Abstract: The different investigation has been carried out on the biological activities of plant extracts but the effect of this natural product on the antibacterial activity of different antibiotics has not been demonstrated. In this study the enhancement effect of Juglans regia on the antibacterial activity of different antibiotics was evaluated against Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). Disk diffusion method was used to determine the antibacterial activity of these antibiotics in the absence and presence of water extract of J. regia. A clinical isolate of E. coli and S. aureus was used as test strain. In the presence of water extract (1000 µg/disc) the antibacterial activities of Amikacin, Imepenem, Aztreonam, Cephalothin, Tobramycin, Amoxiclave, Vancomycin, and Cefotaxime have been increased against test strains. The highest fold increase in area was observed for CTX against S. aureus (a 23 mm increase in inhibition zone). Also the increases in inhibition zone diameter for AK, AT, CEP and CIP were 2, 8, 6 and 3 mm, respectively against E. coli, likewise, improving in diameter of inhibition zone of TB, AMC, VA, and CIP were 10, 6, 15, 12 mm, respectively against S. aureus. Conversely, in case of IMP, J. regia showed an antagonistic effect on the antibacterial activity of this antibiotic against E. coli. Gene expression studies revealed significant decrease of blaZ gene of S. aureus and the bla-CTM gene of E.coli when treated with the combination of the antibiotics and the Juglans regia extract. Suggesting a possible molecular role in down regulating those genes of antibiotic resistance in these pathogens.

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doi:10.23918/ICASEE2018.01

Proceeding Book of ICASEE

ISBN: 978-9922-9036-1-3

5th year , 7 – 9th April, 2018

Conference Issue